Detailed information of TA system TA228859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1020179..1020798
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ70_RS04855
• Protein ID: WP_003404726.1
• Coordinates: 1020364..1020798 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ70_RS04850
• Protein ID: WP_003898641.1
• Coordinates: 1020179..1020358 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS04835 (LVJ70_04830)	1015652..1017232	+	1581	WP_003909313.1	serine hydrolase	-
LVJ70_RS04840 (LVJ70_04835)	1017229..1019622	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ70_RS04845 (LVJ70_04840)	1019895..1020053	+	159	WP_003404720.1	hypothetical protein	-
LVJ70_RS04850 (LVJ70_04845)	1020179..1020358	+	180	WP_003898641.1	antitoxin	Antitoxin
LVJ70_RS04855 (LVJ70_04850)	1020364..1020798	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ70_RS04860 (LVJ70_04855)	1020896..1021669	+	774	WP_003404735.1	VOC family protein	-
LVJ70_RS04865 (LVJ70_04860)	1021734..1022183	+	450	WP_003404738.1	hypothetical protein	-
LVJ70_RS04870 (LVJ70_04865)	1022318..1022548	-	231	WP_003898642.1	hypothetical protein	-
LVJ70_RS04875 (LVJ70_04870)	1022715..1024223	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ70_RS04880 (LVJ70_04875)	1024225..1025463	-	1239	WP_015456182.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T228859 WP_003404726.1 NZ_CP089775:1020364-1020798 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T228859 NZ_CP124351:866680-867054 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT228859 WP_003898641.1 NZ_CP089775:1020179-1020358 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT228859 NZ_CP124351:866223-866591 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5