Detailed information of TA system TA228856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 760442..761234
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: LVJ70_RS03525
• Protein ID: WP_003403386.1
• Coordinates: 760797..761234 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: LVJ70_RS03520
• Protein ID: WP_003403381.1
• Coordinates: 760442..760687 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS03490 (LVJ70_03485)	755462..756967	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LVJ70_RS03495 (LVJ70_03490)	757048..758058	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
LVJ70_RS03500 (LVJ70_03495)	758446..758829	-	384	WP_003403365.1	ribonuclease VapC6	-
LVJ70_RS03505 (LVJ70_03500)	758924..759079	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ70_RS03510 (LVJ70_03505)	759155..759871	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LVJ70_RS03515 (LVJ70_03510)	760147..760455	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
LVJ70_RS03520 (LVJ70_03515)	760442..760687	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
LVJ70_RS03525 (LVJ70_03520)	760797..761234	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ70_RS03530 (LVJ70_03525)	761231..761485	-	255	WP_003911263.1	antitoxin VapB7	-
LVJ70_RS03535 (LVJ70_03530)	761599..763962	+	2364	WP_003898547.1	arylsulfatase AtsD	-
LVJ70_RS03540 (LVJ70_03535)	764025..764351	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ70_RS03545 (LVJ70_03540)	764263..764601	+	339	WP_003403405.1	PIN domain-containing protein	-
LVJ70_RS03550 (LVJ70_03545)	764598..764771	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T228856 WP_003403386.1 NZ_CP089775:c761234-760797 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T228856 NZ_CP124350:95734-96150 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT228856 WP_003403381.1 NZ_CP089775:c760687-760442 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT228856 NZ_CP124350:95507-95737 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0