Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 760147..760687 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag LVJ70_RS03515 Protein ID WP_003403376.1
Coordinates 760147..760455 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ70_RS03520 Protein ID WP_003403381.1
Coordinates 760442..760687 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS03490 (LVJ70_03485) 755462..756967 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ70_RS03495 (LVJ70_03490) 757048..758058 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ70_RS03500 (LVJ70_03495) 758446..758829 - 384 WP_003403365.1 ribonuclease VapC6 -
LVJ70_RS03505 (LVJ70_03500) 758924..759079 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ70_RS03510 (LVJ70_03505) 759155..759871 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ70_RS03515 (LVJ70_03510) 760147..760455 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ70_RS03520 (LVJ70_03515) 760442..760687 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ70_RS03525 (LVJ70_03520) 760797..761234 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
LVJ70_RS03530 (LVJ70_03525) 761231..761485 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ70_RS03535 (LVJ70_03530) 761599..763962 + 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ70_RS03540 (LVJ70_03535) 764025..764351 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ70_RS03545 (LVJ70_03540) 764263..764601 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ70_RS03550 (LVJ70_03545) 764598..764771 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T228855 WP_003403376.1 NZ_CP089775:c760455-760147 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T228855 NZ_CP124350:c94727-94422 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT228855 WP_003403381.1 NZ_CP089775:c760687-760442 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT228855 NZ_CP124350:c94947-94729 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References