Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 721339..721988 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LVJ70_RS03315 Protein ID WP_003403236.1
Coordinates 721593..721988 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LVJ70_RS03310 Protein ID WP_003403235.1
Coordinates 721339..721593 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS03285 (LVJ70_03280) 716465..716548 + 84 Protein_651 galactose-1-phosphate uridylyltransferase -
LVJ70_RS03290 (LVJ70_03285) 716567..717648 + 1082 Protein_652 galactose-1-phosphate uridylyltransferase -
LVJ70_RS03295 (LVJ70_03290) 717645..718736 + 1092 WP_003906403.1 galactokinase -
LVJ70_RS03300 (LVJ70_03295) 719131..720195 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ70_RS03305 (LVJ70_03300) 720299..721246 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ70_RS03310 (LVJ70_03305) 721339..721593 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LVJ70_RS03315 (LVJ70_03310) 721593..721988 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LVJ70_RS03320 (LVJ70_03315) 722082..722822 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ70_RS03325 (LVJ70_03320) 722954..723214 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ70_RS03330 (LVJ70_03325) 723211..723618 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LVJ70_RS03335 (LVJ70_03330) 723690..724841 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ70_RS03340 (LVJ70_03335) 724934..726661 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T228852 WP_003403236.1 NZ_CP089775:721593-721988 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T228852 NZ_CP124349:4360818-4361120 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT228852 WP_003403235.1 NZ_CP089775:721339-721593 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT228852 NZ_CP124349:4361156-4361497 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References