Detailed information of TA system TA022885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 325976..326604
• Replicon: chromosome
• Accession: NC_010718
• Organism: Natranaerobius thermophilus JW/NM-WN-LF

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B2A573
• Locus tag: NTHER_RS01700
• Protein ID: WP_012446795.1
• Coordinates: 326254..326604 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: NTHER_RS01695
• Protein ID: WP_012446794.1
• Coordinates: 325976..326248 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NTHER_RS01675	321285..322886	+	1602	WP_012446790.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
NTHER_RS01680	322952..323395	+	444	WP_012446791.1	CBS domain-containing protein	-
NTHER_RS01685	323463..324488	+	1026	WP_158438188.1	hypothetical protein	-
NTHER_RS01690	324660..325832	+	1173	WP_012446793.1	alanine racemase	-
NTHER_RS01695	325976..326248	+	273	WP_012446794.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NTHER_RS01700	326254..326604	+	351	WP_012446795.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NTHER_RS01705	326660..327667	+	1008	WP_012446796.1	D-cysteine desulfhydrase family protein	-
NTHER_RS01710	327700..328704	+	1005	WP_012446797.1	asparaginase	-
NTHER_RS01715	328751..329566	+	816	WP_012446798.1	M55 family metallopeptidase	-
NTHER_RS01720	329673..330701	+	1029	WP_012446799.1	M42 family metallopeptidase	-
NTHER_RS01725	330821..331453	-	633	WP_012446800.1	protein-L-isoaspartate(D-aspartate) O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-44)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12972.02 Da
• Isoelectric Point: 7.2009

>T22885 WP_012446795.1 NC_010718:326254-326604 [Natranaerobius thermophilus JW/NM-WN-LF]
VNIKRGDIYYADLSPVIGSEQGGVRPVLVVQNDIGNKYSPTVIVVAITSQIQKAKLPTHVEITAEDYNLDKNSVVLTEQL
RTIDKQRLKEKITHIDKELMNKISEAIKISLGLIEF

Nucleotide

• Length: 351 bp

>T22885 NC_010718:326254-326604 [Natranaerobius thermophilus JW/NM-WN-LF]
GTGAATATAAAACGTGGAGATATATATTATGCAGACCTAAGCCCTGTGATCGGTTCAGAACAGGGTGGAGTCAGACCAGT
TTTAGTAGTACAAAATGATATCGGTAACAAGTACAGCCCCACTGTAATCGTAGTAGCAATCACTTCTCAAATTCAAAAAG
CTAAACTTCCGACCCATGTAGAAATAACCGCTGAAGATTATAATTTAGATAAAAATTCAGTTGTATTGACTGAACAACTT
CGCACAATCGATAAACAGCGGTTAAAAGAAAAAATCACCCATATTGATAAAGAATTAATGAATAAAATAAGTGAAGCAAT
TAAGATCAGTCTTGGATTAATAGAATTCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10474.87 Da
• Isoelectric Point: 4.4301

>AT22885 WP_012446794.1 NC_010718:325976-326248 [Natranaerobius thermophilus JW/NM-WN-LF]
MSKTERIMISLPENLLEEIDGIVSNEQCSRSEFVRKAMRLYIEERRKREIRERMQKGYQEMASINLEIANEALEAENEAN
QLAEDIVSGV

Nucleotide

• Length: 273 bp

>AT22885 NC_010718:325976-326248 [Natranaerobius thermophilus JW/NM-WN-LF]
TTGTCAAAAACGGAAAGAATTATGATAAGTTTACCAGAAAATCTACTAGAAGAGATCGACGGTATTGTTTCTAATGAACA
ATGCAGTCGAAGTGAATTTGTCAGAAAAGCTATGCGCTTGTATATAGAGGAGAGGAGAAAAAGAGAAATTCGTGAGAGGA
TGCAAAAGGGTTATCAAGAGATGGCCTCAATCAATTTGGAGATAGCTAATGAAGCACTGGAGGCTGAGAATGAAGCTAAT
CAATTAGCAGAAGATATAGTTAGTGGAGTGTGA

Structures

Toxin

• AlphaFold DB: B2A573