Detailed information of TA system TA228849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 702083..702729
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ70_RS03175
• Protein ID: WP_003403137.1
• Coordinates: 702083..702496 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ70_RS03180
• Protein ID: WP_003403139.1
• Coordinates: 702493..702729 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS03155 (LVJ70_03150)	698166..699716	+	1551	WP_003403119.1	MlaD family protein	-
LVJ70_RS03160 (LVJ70_03155)	699768..700160	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ70_RS03165 (LVJ70_03160)	700157..700414	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ70_RS03170 (LVJ70_03165)	700597..701832	-	1236	WP_003403128.1	ATP-binding protein	-
LVJ70_RS03175 (LVJ70_03170)	702083..702496	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ70_RS03180 (LVJ70_03175)	702493..702729	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ70_RS03185 (LVJ70_03180)	702833..703339	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ70_RS03190 (LVJ70_03185)	703453..703983	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ70_RS03195 (LVJ70_03190)	703967..704662	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ70_RS03200 (LVJ70_03195)	704785..705096	+	312	WP_003403164.1	hypothetical protein	-
LVJ70_RS03205 (LVJ70_03200)	705168..706118	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ70_RS03210 (LVJ70_03205)	706359..706943	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ70_RS03215 (LVJ70_03210)	706945..707655	+	711	Protein_637	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T228849 WP_003403137.1 NZ_CP089775:c702496-702083 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T228849 NZ_CP124349:3880299-3880583 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT228849 WP_003403139.1 NZ_CP089775:c702729-702493 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT228849 NZ_CP124349:3880063-3880296 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89