Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 699768..700414 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LVJ70_RS03160 Protein ID WP_003403122.1
Coordinates 699768..700160 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LVJ70_RS03165 Protein ID WP_003403125.1
Coordinates 700157..700414 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS03145 (LVJ70_03140) 695429..696956 + 1528 Protein_623 virulence factor Mce family protein -
LVJ70_RS03150 (LVJ70_03145) 697019..698161 + 1143 WP_003911253.1 virulence factor Mce family protein -
LVJ70_RS03155 (LVJ70_03150) 698166..699716 + 1551 WP_003403119.1 MlaD family protein -
LVJ70_RS03160 (LVJ70_03155) 699768..700160 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LVJ70_RS03165 (LVJ70_03160) 700157..700414 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LVJ70_RS03170 (LVJ70_03165) 700597..701832 - 1236 WP_003403128.1 ATP-binding protein -
LVJ70_RS03175 (LVJ70_03170) 702083..702496 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LVJ70_RS03180 (LVJ70_03175) 702493..702729 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LVJ70_RS03185 (LVJ70_03180) 702833..703339 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ70_RS03190 (LVJ70_03185) 703453..703983 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LVJ70_RS03195 (LVJ70_03190) 703967..704662 - 696 WP_003918099.1 two-component system response regulator TcrA -
LVJ70_RS03200 (LVJ70_03195) 704785..705096 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T228848 WP_003403122.1 NZ_CP089775:c700160-699768 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T228848 NZ_CP124349:3770028-3770315 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT228848 WP_003403125.1 NZ_CP089775:c700414-700157 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT228848 NZ_CP124349:3770302-3770604 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References