Detailed information of TA system TA228846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 645152..645828
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ70_RS02925
• Protein ID: WP_003898500.1
• Coordinates: 645152..645565 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ70_RS02930
• Protein ID: WP_003402915.1
• Coordinates: 645562..645828 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS02895 (LVJ70_02890)	640497..640799	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ70_RS02900 (LVJ70_02895)	640859..641137	-	279	WP_003402901.1	hypothetical protein	-
LVJ70_RS02905 (LVJ70_02900)	641134..642387	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ70_RS02910 (LVJ70_02905)	642507..642893	-	387	WP_003402907.1	VOC family protein	-
LVJ70_RS02915 (LVJ70_02910)	642956..643840	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ70_RS02920 (LVJ70_02915)	643936..644838	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ70_RS02925 (LVJ70_02920)	645152..645565	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ70_RS02930 (LVJ70_02925)	645562..645828	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ70_RS02935 (LVJ70_02930)	646020..647735	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ70_RS02940 (LVJ70_02935)	647813..649417	+	1605	WP_003402920.1	amidohydrolase	-
LVJ70_RS02945 (LVJ70_02940)	649414..650394	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T228846 WP_003898500.1 NZ_CP089775:c645565-645152 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T228846 NZ_CP124349:3589809-3590159 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT228846 WP_003402915.1 NZ_CP089775:c645828-645562 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT228846 NZ_CP124349:3589564-3589815 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0