Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 72059..72692 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag LVJ70_RS00365 Protein ID WP_003400580.1
Coordinates 72291..72692 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P0CW29
Locus tag LVJ70_RS00360 Protein ID WP_003400577.1
Coordinates 72059..72298 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS00345 (LVJ70_00345) 68260..69699 + 1440 WP_003901784.1 FAD-binding oxidoreductase -
LVJ70_RS00350 (LVJ70_00350) 69755..69916 + 162 WP_003899796.1 hypothetical protein -
LVJ70_RS00355 (LVJ70_00355) 69957..71992 + 2036 Protein_67 UPF0182 family protein -
LVJ70_RS00360 (LVJ70_00360) 72059..72298 + 240 WP_003400577.1 antitoxin VapB1 Antitoxin
LVJ70_RS00365 (LVJ70_00365) 72291..72692 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
LVJ70_RS00370 (LVJ70_00370) 72744..74981 - 2238 WP_003899797.1 NADP-dependent isocitrate dehydrogenase -
LVJ70_RS00375 (LVJ70_00375) 75099..75668 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
LVJ70_RS00380 (LVJ70_00380) 75771..76682 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T228841 WP_003400580.1 NZ_CP089775:72291-72692 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T228841 NZ_CP124349:c2656348-2656241 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 8.5960

>AT228841 WP_003400577.1 NZ_CP089775:72059-72298 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT228841 NZ_CP124349:2656405-2656462 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHM7

References