Detailed information of TA system TA228840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3823258..3823943
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: LVJ69_RS18005
• Protein ID: WP_003417998.1
• Coordinates: 3823533..3823943 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LVJ69_RS18000
• Protein ID: WP_003912220.1
• Coordinates: 3823258..3823536 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS17980 (LVJ69_17980)	3819247..3820848	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
LVJ69_RS17985 (LVJ69_17985)	3820865..3821569	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
LVJ69_RS17990 (LVJ69_17990)	3821687..3822253	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
LVJ69_RS17995 (LVJ69_17995)	3822315..3823202	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
LVJ69_RS18000 (LVJ69_18000)	3823258..3823536	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ69_RS18005 (LVJ69_18005)	3823533..3823943	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ69_RS18010 (LVJ69_18010)	3823976..3825712	-	1737	WP_003418002.1	cholesterol oxidase	-
LVJ69_RS18015 (LVJ69_18015)	3825768..3826895	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
LVJ69_RS18020 (LVJ69_18020)	3826915..3828504	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T228840 WP_003417998.1 NZ_CP089774:3823533-3823943 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T228840 NZ_CP124349:c2553850-2553323 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT228840 WP_003912220.1 NZ_CP089774:3823258-3823536 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT228840 NZ_CP124349:c2554123-2553857 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: G0TIR9