Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3180283..3180970 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ69_RS15175 Protein ID WP_003414624.1
Coordinates 3180527..3180970 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ69_RS15170 Protein ID WP_003414620.1
Coordinates 3180283..3180540 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS15150 (LVJ69_15150) 3175603..3176457 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ69_RS15155 (LVJ69_15155) 3176513..3177676 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ69_RS15160 (LVJ69_15160) 3177693..3178907 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ69_RS15165 (LVJ69_15165) 3178915..3180156 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ69_RS15170 (LVJ69_15170) 3180283..3180540 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ69_RS15175 (LVJ69_15175) 3180527..3180970 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ69_RS15180 (LVJ69_15180) 3181050..3181712 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ69_RS15185 (LVJ69_15185) 3181808..3181999 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ69_RS15190 (LVJ69_15190) 3182331..3184079 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
LVJ69_RS15195 (LVJ69_15195) 3184175..3184756 + 582 WP_003900589.1 fasciclin domain-containing protein -
LVJ69_RS15200 (LVJ69_15200) 3184856..3185122 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T228835 WP_003414624.1 NZ_CP089774:3180527-3180970 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T228835 NZ_CP124349:c919187-918936 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT228835 WP_003414620.1 NZ_CP089774:3180283-3180540 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT228835 NZ_CP124349:c918939-918685 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References