Detailed information of TA system TA228829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3064975..3065654
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: LVJ69_RS14560
• Protein ID: WP_003414059.1
• Coordinates: 3064975..3065391 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LVJ69_RS14565
• Protein ID: WP_003414061.1
• Coordinates: 3065388..3065654 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS14540 (LVJ69_14540)	3061027..3061929	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LVJ69_RS14545 (LVJ69_14545)	3061998..3062750	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LVJ69_RS14550 (LVJ69_14555)	3062994..3063269	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LVJ69_RS14555 (LVJ69_14560)	3063266..3064888	-	1623	WP_003414057.1	class I SAM-dependent DNA methyltransferase	-
LVJ69_RS14560 (LVJ69_14565)	3064975..3065391	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
LVJ69_RS14565 (LVJ69_14570)	3065388..3065654	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ69_RS14570 (LVJ69_14575)	3065680..3066075	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
LVJ69_RS14575 (LVJ69_14580)	3066072..3066341	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ69_RS14580 (LVJ69_14585)	3066351..3067445	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
LVJ69_RS14585 (LVJ69_14590)	3067442..3067861	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LVJ69_RS14590 (LVJ69_14595)	3067860..3067934	+	75	Protein_2879	hypothetical protein	-
LVJ69_RS14595 (LVJ69_14600)	3067935..3068414	-	480	WP_003414073.1	dihydrofolate reductase	-
LVJ69_RS14600 (LVJ69_14605)	3068485..3069285	-	801	WP_003911953.1	thymidylate synthase	-
LVJ69_RS14605 (LVJ69_14610)	3069441..3070178	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T228829 WP_003414059.1 NZ_CP089774:c3065391-3064975 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T228829 NZ_CP124347:c4549032-4548859 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT228829 WP_003414061.1 NZ_CP089774:c3065654-3065388 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT228829 NZ_CP124347:c4549356-4549060 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64