Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2865734..2866371 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ69_RS13470 Protein ID WP_003413180.1
Coordinates 2865734..2866129 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ69_RS13475 Protein ID WP_003413183.1
Coordinates 2866126..2866371 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS13430 (LVJ69_13430) 2861819..2862478 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ69_RS13435 (LVJ69_13435) 2862475..2863134 + 660 WP_003900847.1 LppA family lipoprotein -
LVJ69_RS13440 (LVJ69_13440) 2863131..2863793 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ69_RS13445 (LVJ69_13445) 2863790..2864068 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ69_RS13450 (LVJ69_13450) 2864161..2864574 + 414 WP_003413164.1 PIN domain nuclease -
LVJ69_RS13455 (LVJ69_13455) 2864613..2864870 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ69_RS13460 (LVJ69_13460) 2864867..2865244 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ69_RS13465 (LVJ69_13465) 2865260..2865634 - 375 WP_003413177.1 hypothetical protein -
LVJ69_RS13470 (LVJ69_13470) 2865734..2866129 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ69_RS13475 (LVJ69_13475) 2866126..2866371 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ69_RS13480 (LVJ69_13480) 2866782..2867201 - 420 WP_003413190.1 A24 family peptidase -
LVJ69_RS13485 (LVJ69_13485) 2867213..2868022 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ69_RS13490 (LVJ69_13490) 2868019..2869272 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ69_RS13495 (LVJ69_13495) 2869265..2869777 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T228826 WP_003413180.1 NZ_CP089774:c2866129-2865734 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T228826 NZ_CP124347:c4154714-4154427 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT228826 WP_003413183.1 NZ_CP089774:c2866371-2866126 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT228826 NZ_CP124347:c4154440-4154138 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References