Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2864613..2865244 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ69_RS13460 Protein ID WP_003413174.1
Coordinates 2864867..2865244 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ69_RS13455 Protein ID WP_003413167.1
Coordinates 2864613..2864870 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS13420 (LVJ69_13420) 2859871..2860185 + 315 WP_009937839.1 hypothetical protein -
LVJ69_RS13425 (LVJ69_13425) 2860481..2861692 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ69_RS13430 (LVJ69_13430) 2861819..2862478 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ69_RS13435 (LVJ69_13435) 2862475..2863134 + 660 WP_003900847.1 LppA family lipoprotein -
LVJ69_RS13440 (LVJ69_13440) 2863131..2863793 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ69_RS13445 (LVJ69_13445) 2863790..2864068 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ69_RS13450 (LVJ69_13450) 2864161..2864574 + 414 WP_003413164.1 PIN domain nuclease -
LVJ69_RS13455 (LVJ69_13455) 2864613..2864870 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ69_RS13460 (LVJ69_13460) 2864867..2865244 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ69_RS13465 (LVJ69_13465) 2865260..2865634 - 375 WP_003413177.1 hypothetical protein -
LVJ69_RS13470 (LVJ69_13470) 2865734..2866129 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ69_RS13475 (LVJ69_13475) 2866126..2866371 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ69_RS13480 (LVJ69_13480) 2866782..2867201 - 420 WP_003413190.1 A24 family peptidase -
LVJ69_RS13485 (LVJ69_13485) 2867213..2868022 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ69_RS13490 (LVJ69_13490) 2868019..2869272 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ69_RS13495 (LVJ69_13495) 2869265..2869777 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T228825 WP_003413174.1 NZ_CP089774:2864867-2865244 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T228825 NZ_CP124347:c4044977-4044663 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT228825 WP_003413167.1 NZ_CP089774:2864613-2864870 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT228825 NZ_CP124347:c4045213-4044980 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References