Detailed information of TA system TA228823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2849618..2850258
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ69_RS13360
• Protein ID: WP_003412970.1
• Coordinates: 2849618..2850037 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ69_RS13365
• Protein ID: WP_003412975.1
• Coordinates: 2850034..2850258 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS13330 (LVJ69_13330)	2845203..2845925	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ69_RS13335 (LVJ69_13335)	2846442..2846669	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ69_RS13340 (LVJ69_13340)	2846666..2847067	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ69_RS13345 (LVJ69_13345)	2847102..2848022	-	921	WP_003412965.1	restriction endonuclease	-
LVJ69_RS13350 (LVJ69_13350)	2848363..2848608	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ69_RS13355 (LVJ69_13355)	2848667..2849617	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ69_RS13360 (LVJ69_13360)	2849618..2850037	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ69_RS13365 (LVJ69_13365)	2850034..2850258	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ69_RS13370 (LVJ69_13370)	2850289..2853132	-	2844	WP_031649732.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LVJ69_RS13375 (LVJ69_13375)	2853204..2853605	-	402	WP_003412981.1	hypothetical protein	-
LVJ69_RS13380 (LVJ69_13380)	2853605..2854075	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ69_RS13385 (LVJ69_13385)	2854078..2854641	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T228823 WP_003412970.1 NZ_CP089774:c2850037-2849618 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T228823 NZ_CP124347:3631504-3631722 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT228823 WP_003412975.1 NZ_CP089774:c2850258-2850034 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT228823 NZ_CP124347:3631104-3631478 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09