Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2803434..2804086 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID Q7D717
Locus tag LVJ69_RS13165 Protein ID WP_003900860.1
Coordinates 2803661..2804086 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag LVJ69_RS13160 Protein ID WP_003412749.1
Coordinates 2803434..2803655 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS13145 (LVJ69_13145) 2801674..2801880 - 207 WP_003899347.1 hypothetical protein -
LVJ69_RS13150 (LVJ69_13150) 2802016..2802639 + 624 WP_003900858.1 TIGR00725 family protein -
LVJ69_RS13155 (LVJ69_13155) 2802629..2803381 + 753 WP_003900859.1 hypothetical protein -
LVJ69_RS13160 (LVJ69_13160) 2803434..2803655 + 222 WP_003412749.1 antitoxin Antitoxin
LVJ69_RS13165 (LVJ69_13165) 2803661..2804086 + 426 WP_003900860.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ69_RS13170 (LVJ69_13170) 2804109..2805290 - 1182 WP_003899351.1 dihydrolipoamide acetyltransferase family protein -
LVJ69_RS13175 (LVJ69_13175) 2805287..2806333 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
LVJ69_RS13180 (LVJ69_13180) 2806344..2807447 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
LVJ69_RS13185 (LVJ69_13185) 2807706..2808527 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
LVJ69_RS13190 (LVJ69_13190) 2808524..2809081 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(2-139)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15333.82 Da        Isoelectric Point: 8.1220

>T228822 WP_003900860.1 NZ_CP089774:2803661-2804086 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARPVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T228822 NZ_CP124347:c3602561-3602259 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT228822 WP_003412749.1 NZ_CP089774:2803434-2803655 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT228822 NZ_CP124347:c3602223-3601882 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7ZFR7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References