Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2255451..2256077 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag LVJ69_RS10580 Protein ID WP_003410075.1
Coordinates 2255679..2256077 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag LVJ69_RS10575 Protein ID WP_003911750.1
Coordinates 2255451..2255678 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS10565 (LVJ69_10575) 2253490..2253834 - 345 WP_003410065.1 ferredoxin family protein -
LVJ69_RS10570 (LVJ69_10580) 2254023..2255192 - 1170 WP_003899126.1 ATP-binding protein -
LVJ69_RS10575 (LVJ69_10585) 2255451..2255678 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ69_RS10580 (LVJ69_10590) 2255679..2256077 + 399 WP_003410075.1 PIN domain nuclease Toxin
LVJ69_RS10585 (LVJ69_10595) 2256260..2256691 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
LVJ69_RS10590 (LVJ69_10600) 2256792..2257226 + 435 WP_003914358.1 DUF1398 domain-containing protein -
LVJ69_RS10595 (LVJ69_10605) 2257663..2257842 - 180 Protein_2094 hypothetical protein -
LVJ69_RS10600 (LVJ69_10610) 2257930..2258550 + 621 WP_003410086.1 IS110 family transposase -
LVJ69_RS10605 (LVJ69_10615) 2258504..2259094 + 591 WP_003899131.1 IS110 family transposase -
LVJ69_RS10610 (LVJ69_10620) 2259222..2260477 - 1256 Protein_2097 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T228817 WP_003410075.1 NZ_CP089774:2255679-2256077 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T228817 NZ_CP124347:c2024031-2023657 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT228817 WP_003911750.1 NZ_CP089774:2255451-2255678 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT228817 NZ_CP124347:c2024488-2024120 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References