Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1769172..1769785 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ69_RS08330 Protein ID WP_003407786.1
Coordinates 1769381..1769785 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ69_RS08325 Protein ID WP_009935474.1
Coordinates 1769172..1769375 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS08295 (LVJ69_08300) 1764577..1764957 + 381 WP_003407768.1 fumarate reductase subunit C -
LVJ69_RS08300 (LVJ69_08305) 1764954..1765331 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ69_RS08305 (LVJ69_08310) 1765399..1766007 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
LVJ69_RS08310 (LVJ69_08315) 1766191..1767339 + 1149 Protein_1639 MMPL family transporter -
LVJ69_RS08315 (LVJ69_08320) 1767349..1767795 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
LVJ69_RS08320 (LVJ69_08325) 1767830..1769119 + 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ69_RS08325 (LVJ69_08330) 1769172..1769375 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ69_RS08330 (LVJ69_08335) 1769381..1769785 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ69_RS08335 (LVJ69_08340) 1769802..1771544 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ69_RS08340 (LVJ69_08345) 1771537..1773834 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T228808 WP_003407786.1 NZ_CP089774:1769381-1769785 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T228808 NZ_CP124347:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT228808 WP_009935474.1 NZ_CP089774:1769172-1769375 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT228808 NZ_CP124347:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References