Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1690672..1691288 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ69_RS07985 Protein ID WP_003407593.1
Coordinates 1690971..1691288 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ69_RS07980 Protein ID WP_003900349.1
Coordinates 1690672..1690974 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS07970 (LVJ69_07970) 1686558..1688405 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LVJ69_RS07975 (LVJ69_07975) 1688406..1690658 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ69_RS07980 (LVJ69_07980) 1690672..1690974 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ69_RS07985 (LVJ69_07985) 1690971..1691288 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ69_RS07990 (LVJ69_07990) 1691285..1692289 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ69_RS07995 (LVJ69_07995) 1692342..1693631 + 1290 WP_015345075.1 serine hydrolase domain-containing protein -
LVJ69_RS08000 (LVJ69_08000) 1693704..1694429 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
LVJ69_RS08005 (LVJ69_08005) 1694535..1694747 - 213 WP_003898900.1 dodecin family protein -
LVJ69_RS08010 (LVJ69_08010) 1694808..1695206 + 399 WP_003900351.1 hypothetical protein -
LVJ69_RS08015 (LVJ69_08015) 1695251..1696279 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T228807 WP_003407593.1 NZ_CP089774:1690971-1691288 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T228807 NZ_CP124346:c142269-141964 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT228807 WP_003900349.1 NZ_CP089774:1690672-1690974 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT228807 NZ_CP124346:c142489-142271 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References