Detailed information of TA system TA228804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1395482..1396041
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ69_RS06690
• Protein ID: WP_003898789.1
• Coordinates: 1395482..1395775 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ69_RS06695
• Protein ID: WP_003406322.1
• Coordinates: 1395772..1396041 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS06665 (LVJ69_06655)	1391075..1391335	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ69_RS06670 (LVJ69_06660)	1391332..1391763	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ69_RS06675 (LVJ69_06665)	1391786..1393474	-	1689	WP_003910308.1	PE family protein	-
LVJ69_RS06680 (LVJ69_06670)	1393654..1394514	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ69_RS06685 (LVJ69_06675)	1394595..1395425	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ69_RS06690 (LVJ69_06680)	1395482..1395775	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ69_RS06695 (LVJ69_06685)	1395772..1396041	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ69_RS06700 (LVJ69_06690)	1396154..1399849	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ69_RS06705 (LVJ69_06695)	1399991..1400779	-	789	WP_003900303.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T228804 WP_003898789.1 NZ_CP089774:c1395775-1395482 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T228804 NZ_CP124345:c4226919-4226542 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT228804 WP_003406322.1 NZ_CP089774:c1396041-1395772 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT228804 NZ_CP124345:c4227376-4227008 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2