Detailed information of TA system TA228803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1391075..1391763
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ69_RS06670
• Protein ID: WP_003406304.1
• Coordinates: 1391332..1391763 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ69_RS06665
• Protein ID: WP_003406302.1
• Coordinates: 1391075..1391335 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS06645 (LVJ69_06635)	1386652..1387476	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ69_RS06650 (LVJ69_06640)	1387481..1388662	+	1182	WP_070900236.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ69_RS06655 (LVJ69_06645)	1388739..1389839	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ69_RS06660 (LVJ69_06650)	1390010..1390999	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ69_RS06665 (LVJ69_06655)	1391075..1391335	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ69_RS06670 (LVJ69_06660)	1391332..1391763	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ69_RS06675 (LVJ69_06665)	1391786..1393474	-	1689	WP_003910308.1	PE family protein	-
LVJ69_RS06680 (LVJ69_06670)	1393654..1394514	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ69_RS06685 (LVJ69_06675)	1394595..1395425	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ69_RS06690 (LVJ69_06680)	1395482..1395775	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ69_RS06695 (LVJ69_06685)	1395772..1396041	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228803 WP_003406304.1 NZ_CP089774:1391332-1391763 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228803 NZ_CP124345:c4156262-4155975 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228803 WP_003406302.1 NZ_CP089774:1391075-1391335 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228803 NZ_CP124345:c4155988-4155686 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6