Detailed information of TA system TA228802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1246278..1246846
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ69_RS05995
• Protein ID: WP_003405865.1
• Coordinates: 1246472..1246846 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ69_RS05990
• Protein ID: WP_003405863.1
• Coordinates: 1246278..1246475 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS05970 (LVJ69_05965)	1242319..1242957	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ69_RS05975 (LVJ69_05970)	1243047..1244054	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ69_RS05980 (LVJ69_05975)	1244071..1245054	-	984	WP_003898731.1	hypothetical protein	-
LVJ69_RS05985 (LVJ69_05980)	1245117..1246190	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ69_RS05990 (LVJ69_05985)	1246278..1246475	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ69_RS05995 (LVJ69_05990)	1246472..1246846	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ69_RS06000 (LVJ69_05995)	1247049..1247747	+	699	WP_003898733.1	hypothetical protein	-
LVJ69_RS06005 (LVJ69_06000)	1247865..1248050	+	186	WP_003901093.1	hypothetical protein	-
LVJ69_RS06010 (LVJ69_06005)	1247977..1248252	-	276	WP_003405867.1	hypothetical protein	-
LVJ69_RS06015 (LVJ69_06010)	1248495..1248818	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ69_RS06020 (LVJ69_06015)	1248833..1249531	-	699	WP_031646953.1	hypothetical protein	-
LVJ69_RS06025 (LVJ69_06020)	1249565..1249774	+	210	WP_003911400.1	hypothetical protein	-
LVJ69_RS06030 (LVJ69_06025)	1249726..1250496	-	771	Protein_1188	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228802 WP_003405865.1 NZ_CP089774:1246472-1246846 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228802 NZ_CP124345:c4046525-4046211 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228802 WP_003405863.1 NZ_CP089774:1246278-1246475 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228802 NZ_CP124345:c4046761-4046528 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07