Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1029709..1030682 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LVJ69_RS04910 Protein ID WP_003404756.1
Coordinates 1030182..1030682 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID O05910
Locus tag LVJ69_RS04905 Protein ID WP_003898647.1
Coordinates 1029709..1030185 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS04885 (LVJ69_04880) 1025556..1026827 - 1272 WP_003404747.1 PPE family protein -
LVJ69_RS04890 (LVJ69_04885) 1026842..1027141 - 300 WP_003404748.1 PE family protein -
LVJ69_RS04895 (LVJ69_04890) 1027585..1029366 + 1782 WP_003404749.1 BCCT family transporter -
LVJ69_RS04900 (LVJ69_04895) 1029366..1029620 + 255 WP_003898646.1 hypothetical protein -
LVJ69_RS04905 (LVJ69_04900) 1029709..1030185 + 477 WP_003898647.1 DUF1778 domain-containing protein Antitoxin
LVJ69_RS04910 (LVJ69_04905) 1030182..1030682 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LVJ69_RS04920 (LVJ69_04915) 1030995..1032314 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
LVJ69_RS04925 (LVJ69_04920) 1032602..1033183 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LVJ69_RS04930 (LVJ69_04925) 1033183..1034835 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T228799 WP_003404756.1 NZ_CP089774:1030182-1030682 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T228799 NZ_CP124345:c3604110-3603808 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17387.98 Da        Isoelectric Point: 11.2467

>AT228799 WP_003898647.1 NZ_CP089774:1029709-1030185 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT228799 NZ_CP124345:c3603772-3603431 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB A5U0V8

References