Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 760443..761235 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag LVJ69_RS03525 Protein ID WP_003403386.1
Coordinates 760798..761235 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ69_RS03520 Protein ID WP_003403381.1
Coordinates 760443..760688 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS03490 (LVJ69_03485) 755463..756968 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ69_RS03495 (LVJ69_03490) 757049..758059 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ69_RS03500 (LVJ69_03495) 758447..758830 - 384 WP_003403365.1 ribonuclease VapC6 -
LVJ69_RS03505 (LVJ69_03500) 758925..759080 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ69_RS03510 (LVJ69_03505) 759156..759872 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ69_RS03515 (LVJ69_03510) 760148..760456 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ69_RS03520 (LVJ69_03515) 760443..760688 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ69_RS03525 (LVJ69_03520) 760798..761235 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ69_RS03530 (LVJ69_03525) 761232..761486 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ69_RS03535 (LVJ69_03530) 761600..763963 + 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ69_RS03540 (LVJ69_03535) 764026..764352 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ69_RS03545 (LVJ69_03540) 764264..764602 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ69_RS03550 (LVJ69_03545) 764599..764772 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T228795 WP_003403386.1 NZ_CP089774:c761235-760798 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T228795 NZ_CP124345:2268362-2268464 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT228795 WP_003403381.1 NZ_CP089774:c760688-760443 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT228795 NZ_CP124345:c2268502-2268234 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References