Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 758447..759080 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag LVJ69_RS03500 Protein ID WP_003403365.1
Coordinates 758447..758830 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag LVJ69_RS03505 Protein ID WP_003403368.1
Coordinates 758925..759080 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS03475 (LVJ69_03470) 753739..754275 + 537 WP_003403341.1 50S ribosomal protein L10 -
LVJ69_RS03480 (LVJ69_03475) 754312..754704 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
LVJ69_RS03485 (LVJ69_03480) 754697..755392 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
LVJ69_RS03490 (LVJ69_03485) 755463..756968 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ69_RS03495 (LVJ69_03490) 757049..758059 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ69_RS03500 (LVJ69_03495) 758447..758830 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
LVJ69_RS03505 (LVJ69_03500) 758925..759080 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ69_RS03510 (LVJ69_03505) 759156..759872 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ69_RS03515 (LVJ69_03510) 760148..760456 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ69_RS03520 (LVJ69_03515) 760443..760688 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
LVJ69_RS03525 (LVJ69_03520) 760798..761235 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
LVJ69_RS03530 (LVJ69_03525) 761232..761486 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ69_RS03535 (LVJ69_03530) 761600..763963 + 2364 WP_003898547.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T228793 WP_003403365.1 NZ_CP089774:c758830-758447 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T228793 NZ_CP124345:2012253-2012507 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT228793 WP_003403368.1 NZ_CP089774:c759080-758925 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT228793 NZ_CP124345:2012005-2012256 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References