Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 721340..721989 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag LVJ69_RS03315 Protein ID WP_003403236.1
Coordinates 721594..721989 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag LVJ69_RS03310 Protein ID WP_003403235.1
Coordinates 721340..721594 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS03285 (LVJ69_03280) 716466..716549 + 84 Protein_651 galactose-1-phosphate uridylyltransferase -
LVJ69_RS03290 (LVJ69_03285) 716568..717649 + 1082 Protein_652 galactose-1-phosphate uridylyltransferase -
LVJ69_RS03295 (LVJ69_03290) 717646..718737 + 1092 WP_003906403.1 galactokinase -
LVJ69_RS03300 (LVJ69_03295) 719132..720196 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ69_RS03305 (LVJ69_03300) 720300..721247 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ69_RS03310 (LVJ69_03305) 721340..721594 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
LVJ69_RS03315 (LVJ69_03310) 721594..721989 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
LVJ69_RS03320 (LVJ69_03315) 722083..722823 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ69_RS03325 (LVJ69_03320) 722955..723215 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ69_RS03330 (LVJ69_03325) 723212..723619 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
LVJ69_RS03335 (LVJ69_03330) 723691..724842 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ69_RS03340 (LVJ69_03335) 724935..726662 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T228791 WP_003403236.1 NZ_CP089774:721594-721989 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T228791 NZ_CP124345:977677-978084 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT228791 WP_003403235.1 NZ_CP089774:721340-721594 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT228791 NZ_CP124345:977430-977696 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References