Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 645153..645829 Replicon chromosome
Accession NZ_CP089774
Organism Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag LVJ69_RS02925 Protein ID WP_003898500.1
Coordinates 645153..645566 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag LVJ69_RS02930 Protein ID WP_003402915.1
Coordinates 645563..645829 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ69_RS02895 (LVJ69_02890) 640498..640800 - 303 WP_003402896.1 DUF3349 domain-containing protein -
LVJ69_RS02900 (LVJ69_02895) 640860..641138 - 279 WP_003402901.1 hypothetical protein -
LVJ69_RS02905 (LVJ69_02900) 641135..642388 - 1254 WP_003402904.1 inorganic phosphate transporter -
LVJ69_RS02910 (LVJ69_02905) 642508..642894 - 387 WP_003402907.1 VOC family protein -
LVJ69_RS02915 (LVJ69_02910) 642957..643841 - 885 WP_003402909.1 SDR family oxidoreductase -
LVJ69_RS02920 (LVJ69_02915) 643937..644839 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
LVJ69_RS02925 (LVJ69_02920) 645153..645566 - 414 WP_003898500.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ69_RS02930 (LVJ69_02925) 645563..645829 - 267 WP_003402915.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LVJ69_RS02935 (LVJ69_02930) 646021..647736 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
LVJ69_RS02940 (LVJ69_02935) 647814..649418 + 1605 WP_003402920.1 amidohydrolase -
LVJ69_RS02945 (LVJ69_02940) 649415..650395 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T228785 WP_003898500.1 NZ_CP089774:c645566-645153 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T228785 NZ_CP124345:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT228785 WP_003402915.1 NZ_CP089774:c645829-645563 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT228785 NZ_CP124345:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References