Detailed information of TA system TA228781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 366868..367394
• Replicon: chromosome
• Accession: NZ_CP089774
• Organism: Mycobacterium tuberculosis strain 02-R0894

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ69_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 367092..367394 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: LVJ69_RS01590
• Protein ID: WP_003401555.1
• Coordinates: 366868..367095 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ69_RS01575 (LVJ69_01575)	362561..363364	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ69_RS01580 (LVJ69_01580)	363374..364771	-	1398	WP_003401544.1	sulfatase	-
LVJ69_RS01585 (LVJ69_01585)	364950..366725	+	1776	WP_010886074.1	PE family protein	-
LVJ69_RS01590 (LVJ69_01590)	366868..367095	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
LVJ69_RS01595 (LVJ69_01595)	367092..367394	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ69_RS01600 (LVJ69_01600)	367442..367663	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
LVJ69_RS01605 (LVJ69_01605)	367660..368085	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ69_RS01610 (LVJ69_01610)	368221..368853	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ69_RS01615 (LVJ69_01615)	368850..369758	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ69_RS01620 (LVJ69_01620)	369766..370356	-	591	WP_229298012.1	hypothetical protein	-
LVJ69_RS01625 (LVJ69_01625)	370349..370399	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T228781 WP_003401560.1 NZ_CP089774:367092-367394 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T228781 NZ_CP124343:c4227043-4226666 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT228781 WP_003401555.1 NZ_CP089774:366868-367095 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT228781 NZ_CP124343:c4227500-4227132 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1