Detailed information of TA system TA228779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3823005..3823690
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: LVJ68_RS18065
• Protein ID: WP_003417998.1
• Coordinates: 3823280..3823690 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LVJ68_RS18060
• Protein ID: WP_003912220.1
• Coordinates: 3823005..3823283 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS18040 (LVJ68_18040)	3818994..3820595	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
LVJ68_RS18045 (LVJ68_18045)	3820612..3821316	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
LVJ68_RS18050 (LVJ68_18050)	3821434..3822000	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
LVJ68_RS18055 (LVJ68_18055)	3822062..3822949	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
LVJ68_RS18060 (LVJ68_18060)	3823005..3823283	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ68_RS18065 (LVJ68_18065)	3823280..3823690	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS18070 (LVJ68_18070)	3823723..3825459	-	1737	WP_003418002.1	cholesterol oxidase	-
LVJ68_RS18075 (LVJ68_18075)	3825515..3826642	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
LVJ68_RS18080 (LVJ68_18080)	3826662..3828251	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T228779 WP_003417998.1 NZ_CP089773:3823280-3823690 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T228779 NZ_CP124343:c4046649-4046335 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT228779 WP_003912220.1 NZ_CP089773:3823005-3823283 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT228779 NZ_CP124343:c4046885-4046652 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G0TIR9