Detailed information of TA system TA228772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3139580..3140184
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: LVJ68_RS15005
• Protein ID: WP_003414492.1
• Coordinates: 3139580..3139972 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: LVJ68_RS15010
• Protein ID: WP_003414495.1
• Coordinates: 3139969..3140184 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS14975 (LVJ68_14980)	3134730..3135518	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
LVJ68_RS14980 (LVJ68_14985)	3135852..3136397	-	546	WP_003913758.1	DUF1802 family protein	-
LVJ68_RS14985 (LVJ68_14990)	3136669..3137553	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LVJ68_RS14990 (LVJ68_14995)	3137556..3138443	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
LVJ68_RS14995 (LVJ68_15000)	3138748..3139293	-	546	WP_003913758.1	DUF1802 family protein	-
LVJ68_RS15000 (LVJ68_15005)	3139290..3139559	-	270	WP_003414489.1	DUF2277 family protein	-
LVJ68_RS15005 (LVJ68_15010)	3139580..3139972	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS15010 (LVJ68_15015)	3139969..3140184	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ68_RS15015 (LVJ68_15020)	3140231..3140980	+	750	WP_003414497.1	enoyl-CoA hydratase	-
LVJ68_RS15020 (LVJ68_15025)	3141059..3142141	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
LVJ68_RS15025 (LVJ68_15030)	3142134..3143444	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
LVJ68_RS15030 (LVJ68_15035)	3143447..3144274	-	828	Protein_2967	carbohydrate ABC transporter permease	-
LVJ68_RS15035 (LVJ68_15040)	3144271..3145182	-	912	WP_070902957.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T228772 WP_003414492.1 NZ_CP089773:c3139972-3139580 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T228772 NZ_CP124343:2268659-2268761 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT228772 WP_003414495.1 NZ_CP089773:c3140184-3139969 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT228772 NZ_CP124343:c2268799-2268531 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8