Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3139580..3140184 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ68_RS15005 Protein ID WP_003414492.1
Coordinates 3139580..3139972 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ68_RS15010 Protein ID WP_003414495.1
Coordinates 3139969..3140184 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS14975 (LVJ68_14980) 3134730..3135518 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ68_RS14980 (LVJ68_14985) 3135852..3136397 - 546 WP_003913758.1 DUF1802 family protein -
LVJ68_RS14985 (LVJ68_14990) 3136669..3137553 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ68_RS14990 (LVJ68_14995) 3137556..3138443 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ68_RS14995 (LVJ68_15000) 3138748..3139293 - 546 WP_003913758.1 DUF1802 family protein -
LVJ68_RS15000 (LVJ68_15005) 3139290..3139559 - 270 WP_003414489.1 DUF2277 family protein -
LVJ68_RS15005 (LVJ68_15010) 3139580..3139972 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ68_RS15010 (LVJ68_15015) 3139969..3140184 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ68_RS15015 (LVJ68_15020) 3140231..3140980 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ68_RS15020 (LVJ68_15025) 3141059..3142141 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ68_RS15025 (LVJ68_15030) 3142134..3143444 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LVJ68_RS15030 (LVJ68_15035) 3143447..3144274 - 828 Protein_2967 carbohydrate ABC transporter permease -
LVJ68_RS15035 (LVJ68_15040) 3144271..3145182 - 912 WP_070902957.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T228772 WP_003414492.1 NZ_CP089773:c3139972-3139580 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T228772 NZ_CP124343:2268659-2268761 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT228772 WP_003414495.1 NZ_CP089773:c3140184-3139969 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT228772 NZ_CP124343:c2268799-2268531 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References