Detailed information of TA system TA228770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3111754..3112324
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: LVJ68_RS14850
• Protein ID: WP_003414166.1
• Coordinates: 3111754..3112110 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: LVJ68_RS14855
• Protein ID: WP_003901465.1
• Coordinates: 3112094..3112324 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS14830 (LVJ68_14835)	3107206..3108894	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
LVJ68_RS14835 (LVJ68_14840)	3108898..3109224	-	327	WP_003414157.1	hypothetical protein	-
LVJ68_RS14840 (LVJ68_14845)	3109397..3109984	+	588	WP_003914429.1	DUF3558 family protein	-
LVJ68_RS14845 (LVJ68_14850)	3110003..3111652	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
LVJ68_RS14850 (LVJ68_14855)	3111754..3112110	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
LVJ68_RS14855 (LVJ68_14860)	3112094..3112324	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
LVJ68_RS14860 (LVJ68_14865)	3112367..3113410	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
LVJ68_RS14865 (LVJ68_14870)	3113409..3113876	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
LVJ68_RS14870 (LVJ68_14875)	3113905..3114159	-	255	WP_003917684.1	hypothetical protein	-
LVJ68_RS14875 (LVJ68_14880)	3114307..3114711	+	405	WP_009938577.1	hypothetical protein	-
LVJ68_RS14880 (LVJ68_14885)	3114708..3114899	+	192	WP_003414184.1	hypothetical protein	-
LVJ68_RS14885 (LVJ68_14890)	3115116..3115376	+	261	Protein_2938	transposase	-
LVJ68_RS14890 (LVJ68_14895)	3116486..3116743	+	258	WP_003899489.1	hypothetical protein	-
LVJ68_RS14895 (LVJ68_14900)	3116848..3117159	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T228770 WP_003414166.1 NZ_CP089773:c3112110-3111754 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T228770 NZ_CP124343:2012547-2012801 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT228770 WP_003901465.1 NZ_CP089773:c3112324-3112094 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT228770 NZ_CP124343:2012299-2012550 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3