Detailed information of TA system TA022877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 31468..32045
• Replicon: plasmid pET49
• Accession: NC_010697
• Organism: Erwinia tasmaniensis Et1/99

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2VAZ0
• Locus tag: ETA_RS00415
• Protein ID: WP_012443235.1
• Coordinates: 31743..32045 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ETA_RS00410
• Protein ID: WP_012443234.1
• Coordinates: 31468..31746 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETA_RS00385	26662..27288	+	627	WP_157861757.1	hypothetical protein	-
ETA_RS18920	27441..27707	+	267	WP_071819143.1	hypothetical protein	-
ETA_RS00390	27764..27967	+	204	WP_042958396.1	hypothetical protein	-
ETA_RS00395	27970..28215	+	246	WP_012443231.1	hypothetical protein	-
ETA_RS00400	28233..30494	+	2262	WP_012443232.1	DNA topoisomerase 3	-
ETA_RS00405	30785..31222	+	438	WP_012443233.1	H-NS histone family protein	-
ETA_RS00410	31468..31746	-	279	WP_012443234.1	putative addiction module antidote protein	Antitoxin
ETA_RS00415	31743..32045	-	303	WP_012443235.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ETA_RS00420	32445..32633	-	189	WP_012443236.1	hypothetical protein	-
ETA_RS00425	32673..32885	-	213	WP_012443237.1	hypothetical protein	-
ETA_RS18925	33154..33378	-	225	WP_012443238.1	transcriptional regulator	-
ETA_RS00430	33375..33620	-	246	WP_012443239.1	hypothetical protein	-
ETA_RS00435	33617..34153	-	537	WP_012443240.1	hypothetical protein	-
ETA_RS00440	34284..34808	-	525	WP_042958398.1	DnaJ domain-containing protein	-
ETA_RS00445	35289..35480	+	192	WP_012443242.1	hypothetical protein	-
ETA_RS20120	35549..35716	+	168	WP_167310299.1	hypothetical protein	-
ETA_RS19765	35873..36058	-	186	WP_042958400.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48751	48751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-87)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11357.25 Da
• Isoelectric Point: 10.0083

>T22877 WP_012443235.1 NC_010697:c32045-31743 [Erwinia tasmaniensis Et1/99]
MITVLSTEIFDNWIDVLKDVRAKTKIQARIRRLKNGNSGDIEPVGEGFSEMRIHEGKGYRVYLKKHGKVIVVLLCGGDKS
TQQKDILRAKAIYKEIEGEL

Nucleotide

• Length: 303 bp

>T22877 NC_010697:c32045-31743 [Erwinia tasmaniensis Et1/99]
ATGATAACAGTATTAAGTACAGAAATATTTGATAACTGGATTGATGTCCTGAAAGATGTCAGGGCAAAAACAAAGATTCA
GGCACGAATCCGCAGGCTAAAAAATGGAAATTCCGGCGATATTGAACCTGTGGGCGAAGGGTTTTCAGAAATGCGGATTC
ATGAAGGTAAAGGCTACAGGGTTTATCTGAAGAAACACGGTAAGGTCATTGTTGTTTTGTTGTGTGGTGGCGATAAAAGT
ACACAACAAAAAGATATACTCAGAGCTAAAGCCATTTACAAAGAAATCGAGGGGGAGCTATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10261.80 Da
• Isoelectric Point: 5.6174

>AT22877 WP_012443234.1 NC_010697:c31746-31468 [Erwinia tasmaniensis Et1/99]
MKLREYDIAEHLNSEEEMQMYLNEIMEDGEPSLVLSALGDIARARNMSQLSREVGMSREGLYHALSGKGNPTFSAMMKIT
KALGLKLHFTGA

Nucleotide

• Length: 279 bp

>AT22877 NC_010697:c31746-31468 [Erwinia tasmaniensis Et1/99]
ATGAAACTTAGAGAATATGACATTGCGGAACATTTGAATAGTGAAGAAGAAATGCAAATGTACCTCAATGAAATTATGGA
AGATGGTGAACCGTCATTAGTTCTTTCCGCATTGGGTGATATTGCCAGAGCCAGAAACATGAGCCAGCTTTCAAGAGAAG
TAGGAATGTCCAGAGAAGGGCTATATCATGCGCTATCAGGAAAAGGCAATCCAACATTCAGCGCTATGATGAAAATAACA
AAAGCGCTAGGTTTAAAACTTCACTTCACAGGGGCATAA

Structures

Toxin

• AlphaFold DB: B2VAZ0