Detailed information of TA system TA228769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3072170..3072857
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: LVJ68_RS14630
• Protein ID: WP_003414064.1
• Coordinates: 3072462..3072857 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LVJ68_RS14625
• Protein ID: WP_003414061.1
• Coordinates: 3072170..3072436 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS14600 (LVJ68_14600)	3067809..3068711	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LVJ68_RS14605 (LVJ68_14605)	3068780..3069532	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LVJ68_RS14610 (LVJ68_14615)	3069776..3070051	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LVJ68_RS14615 (LVJ68_14620)	3070048..3071670	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
LVJ68_RS14620 (LVJ68_14625)	3071757..3072173	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
LVJ68_RS14625 (LVJ68_14630)	3072170..3072436	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ68_RS14630 (LVJ68_14635)	3072462..3072857	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS14635 (LVJ68_14640)	3072854..3073123	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ68_RS14640 (LVJ68_14645)	3073133..3074227	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
LVJ68_RS14645 (LVJ68_14650)	3074224..3074643	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LVJ68_RS14650 (LVJ68_14655)	3074642..3074716	+	75	Protein_2891	hypothetical protein	-
LVJ68_RS14655 (LVJ68_14660)	3074717..3075196	-	480	WP_003414073.1	dihydrofolate reductase	-
LVJ68_RS14660 (LVJ68_14665)	3075267..3076067	-	801	WP_003911953.1	thymidylate synthase	-
LVJ68_RS14665 (LVJ68_14670)	3076223..3076960	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T228769 WP_003414064.1 NZ_CP089773:c3072857-3072462 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T228769 NZ_CP124343:2012299-2012550 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT228769 WP_003414061.1 NZ_CP089773:c3072436-3072170 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT228769 NZ_CP124343:2012547-2012801 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64