Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2871396..2872027 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ68_RS13520 Protein ID WP_003413174.1
Coordinates 2871650..2872027 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ68_RS13515 Protein ID WP_003413167.1
Coordinates 2871396..2871653 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS13485 (LVJ68_13485) 2867310..2867624 + 315 WP_009937839.1 hypothetical protein -
LVJ68_RS13490 (LVJ68_13490) 2867920..2869131 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ68_RS13495 (LVJ68_13495) 2869258..2869917 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ68_RS13500 (LVJ68_13500) 2869914..2870576 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ68_RS13505 (LVJ68_13505) 2870573..2870851 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ68_RS13510 (LVJ68_13510) 2870944..2871357 + 414 WP_003912787.1 PIN domain nuclease -
LVJ68_RS13515 (LVJ68_13515) 2871396..2871653 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ68_RS13520 (LVJ68_13520) 2871650..2872027 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ68_RS13525 (LVJ68_13525) 2872043..2872417 - 375 WP_003413177.1 hypothetical protein -
LVJ68_RS13530 (LVJ68_13530) 2872517..2872912 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ68_RS13535 (LVJ68_13535) 2872909..2873154 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ68_RS13540 (LVJ68_13540) 2873565..2873984 - 420 WP_003413190.1 A24 family peptidase -
LVJ68_RS13545 (LVJ68_13545) 2873996..2874805 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ68_RS13550 (LVJ68_13550) 2874802..2876055 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ68_RS13555 (LVJ68_13555) 2876048..2876560 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T228764 WP_003413174.1 NZ_CP089773:2871650-2872027 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T228764 NZ_CP124343:291052-291579 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT228764 WP_003413167.1 NZ_CP089773:2871396-2871653 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT228764 NZ_CP124343:290779-291045 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References