Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2255975..2256601 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag LVJ68_RS10600 Protein ID WP_003410075.1
Coordinates 2256203..2256601 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag LVJ68_RS10595 Protein ID WP_003911750.1
Coordinates 2255975..2256202 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS10585 (LVJ68_10595) 2254014..2254358 - 345 WP_003410065.1 ferredoxin family protein -
LVJ68_RS10590 (LVJ68_10600) 2254547..2255716 - 1170 WP_003899126.1 ATP-binding protein -
LVJ68_RS10595 (LVJ68_10605) 2255975..2256202 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ68_RS10600 (LVJ68_10610) 2256203..2256601 + 399 WP_003410075.1 PIN domain nuclease Toxin
LVJ68_RS10605 (LVJ68_10615) 2256784..2257215 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
LVJ68_RS10610 (LVJ68_10620) 2257316..2257750 + 435 WP_003914358.1 DUF1398 domain-containing protein -
LVJ68_RS10615 (LVJ68_10625) 2258187..2258366 - 180 Protein_2098 hypothetical protein -
LVJ68_RS10620 (LVJ68_10630) 2258454..2259074 + 621 WP_003410086.1 IS110 family transposase -
LVJ68_RS10625 (LVJ68_10635) 2259028..2259618 + 591 WP_003899131.1 IS110 family transposase -
LVJ68_RS10630 (LVJ68_10640) 2259746..2261002 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T228756 WP_003410075.1 NZ_CP089773:2256203-2256601 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T228756 NZ_CP124341:c4016079-4015765 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT228756 WP_003911750.1 NZ_CP089773:2255975-2256202 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT228756 NZ_CP124341:c4016315-4016082 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References