Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2223343..2224031 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag LVJ68_RS10430 Protein ID WP_003409958.1
Coordinates 2223343..2223762 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ68_RS10435 Protein ID WP_003409968.1
Coordinates 2223771..2224031 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS10410 (LVJ68_10420) 2218838..2219686 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ68_RS10415 (LVJ68_10425) 2219649..2221094 - 1446 WP_003899115.1 APC family permease -
LVJ68_RS10420 (LVJ68_10430) 2221273..2221959 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ68_RS10425 (LVJ68_10435) 2222150..2223118 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ68_RS10430 (LVJ68_10440) 2223343..2223762 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ68_RS10435 (LVJ68_10445) 2223771..2224031 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ68_RS10440 (LVJ68_10450) 2224174..2225850 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
LVJ68_RS10445 (LVJ68_10455) 2225838..2226491 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ68_RS10450 (LVJ68_10460) 2226606..2226836 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ68_RS10455 (LVJ68_10465) 2226921..2227832 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ68_RS10460 (LVJ68_10470) 2227941..2228540 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T228753 WP_003409958.1 NZ_CP089773:c2223762-2223343 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T228753 NZ_CP124341:c3575061-3574759 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT228753 WP_003409968.1 NZ_CP089773:c2224031-2223771 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT228753 NZ_CP124341:c3574723-3574382 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References