Detailed information of TA system TA228750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2192573..2193276
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LVJ68_RS10220
• Protein ID: WP_003409778.1
• Coordinates: 2192573..2192902 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LVJ68_RS10225
• Protein ID: WP_003409780.1
• Coordinates: 2192899..2193276 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS10200 (LVJ68_10210)	2188956..2190026	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LVJ68_RS10205 (LVJ68_10215)	2190023..2190538	+	516	WP_003409718.1	flavin reductase family protein	-
LVJ68_RS10210 (LVJ68_10220)	2190535..2191596	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LVJ68_RS10215 (LVJ68_10225)	2191593..2192363	+	771	WP_003409775.1	SDR family oxidoreductase	-
LVJ68_RS10220 (LVJ68_10230)	2192573..2192902	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ68_RS10225 (LVJ68_10235)	2192899..2193276	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LVJ68_RS10230 (LVJ68_10240)	2193273..2193863	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LVJ68_RS10235 (LVJ68_10245)	2193918..2195282	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
LVJ68_RS10240 (LVJ68_10250)	2195437..2195889	-	453	WP_003899095.1	lipoprotein	-
LVJ68_RS10245 (LVJ68_10255)	2195953..2196354	+	402	WP_003409869.1	hypothetical protein	-
LVJ68_RS10250 (LVJ68_10260)	2196347..2196529	-	183	WP_003409870.1	hypothetical protein	-
LVJ68_RS10255 (LVJ68_10265)	2196643..2196993	-	351	WP_003899096.1	hypothetical protein	-
LVJ68_RS10260 (LVJ68_10270)	2197004..2197906	-	903	WP_003899097.1	hypothetical protein	-
LVJ68_RS10265 (LVJ68_10275)	2197927..2198118	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T228750 WP_003409778.1 NZ_CP089773:c2192902-2192573 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T228750 NZ_CP124341:2520962-2522284 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT228750 WP_003409780.1 NZ_CP089773:c2193276-2192899 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT228750 NZ_CP124341:2520696-2520962 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8