Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1772590..1773203 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ68_RS08320 Protein ID WP_003407786.1
Coordinates 1772799..1773203 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ68_RS08315 Protein ID WP_009935474.1
Coordinates 1772590..1772793 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS08285 (LVJ68_08290) 1767995..1768375 + 381 WP_003407768.1 fumarate reductase subunit C -
LVJ68_RS08290 (LVJ68_08295) 1768372..1768749 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ68_RS08295 (LVJ68_08300) 1768817..1769425 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
LVJ68_RS08300 (LVJ68_08305) 1769609..1770757 + 1149 Protein_1637 MMPL family transporter -
LVJ68_RS08305 (LVJ68_08310) 1770767..1771213 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
LVJ68_RS08310 (LVJ68_08315) 1771248..1772537 + 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ68_RS08315 (LVJ68_08320) 1772590..1772793 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ68_RS08320 (LVJ68_08325) 1772799..1773203 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ68_RS08325 (LVJ68_08330) 1773220..1774962 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ68_RS08330 (LVJ68_08335) 1774955..1777252 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T228747 WP_003407786.1 NZ_CP089773:1772799-1773203 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T228747 NZ_CP124341:1983876-1984130 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT228747 WP_009935474.1 NZ_CP089773:1772590-1772793 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT228747 NZ_CP124341:1983628-1983879 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References