Detailed information of TA system TA228747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1772590..1773203
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: LVJ68_RS08320
• Protein ID: WP_003407786.1
• Coordinates: 1772799..1773203 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: LVJ68_RS08315
• Protein ID: WP_009935474.1
• Coordinates: 1772590..1772793 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS08285 (LVJ68_08290)	1767995..1768375	+	381	WP_003407768.1	fumarate reductase subunit C	-
LVJ68_RS08290 (LVJ68_08295)	1768372..1768749	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
LVJ68_RS08295 (LVJ68_08300)	1768817..1769425	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
LVJ68_RS08300 (LVJ68_08305)	1769609..1770757	+	1149	Protein_1637	MMPL family transporter	-
LVJ68_RS08305 (LVJ68_08310)	1770767..1771213	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
LVJ68_RS08310 (LVJ68_08315)	1771248..1772537	+	1290	WP_003407781.1	threonine ammonia-lyase	-
LVJ68_RS08315 (LVJ68_08320)	1772590..1772793	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ68_RS08320 (LVJ68_08325)	1772799..1773203	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
LVJ68_RS08325 (LVJ68_08330)	1773220..1774962	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
LVJ68_RS08330 (LVJ68_08335)	1774955..1777252	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T228747 WP_003407786.1 NZ_CP089773:1772799-1773203 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T228747 NZ_CP124341:1983876-1984130 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT228747 WP_009935474.1 NZ_CP089773:1772590-1772793 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT228747 NZ_CP124341:1983628-1983879 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46