Detailed information of TA system TA228743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1395895..1396454
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ68_RS06670
• Protein ID: WP_003898789.1
• Coordinates: 1395895..1396188 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ68_RS06675
• Protein ID: WP_003406322.1
• Coordinates: 1396185..1396454 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS06645 (LVJ68_06635)	1391488..1391748	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ68_RS06650 (LVJ68_06640)	1391745..1392176	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ68_RS06655 (LVJ68_06645)	1392199..1393887	-	1689	WP_003910308.1	PE family protein	-
LVJ68_RS06660 (LVJ68_06650)	1394067..1394927	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ68_RS06665 (LVJ68_06655)	1395008..1395838	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ68_RS06670 (LVJ68_06660)	1395895..1396188	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ68_RS06675 (LVJ68_06665)	1396185..1396454	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ68_RS06680 (LVJ68_06670)	1396567..1400262	-	3696	WP_003898790.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ68_RS06685 (LVJ68_06675)	1400404..1401192	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T228743 WP_003898789.1 NZ_CP089773:c1396188-1395895 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T228743 NZ_CP124341:645572-645886 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT228743 WP_003406322.1 NZ_CP089773:c1396454-1396185 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT228743 NZ_CP124341:645883-646299 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2