Detailed information of TA system TA228742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1391488..1392176
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ68_RS06650
• Protein ID: WP_003406304.1
• Coordinates: 1391745..1392176 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ68_RS06645
• Protein ID: WP_003406302.1
• Coordinates: 1391488..1391748 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS06625 (LVJ68_06615)	1387065..1387889	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ68_RS06630 (LVJ68_06620)	1387894..1389075	+	1182	WP_070892757.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ68_RS06635 (LVJ68_06625)	1389152..1390252	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ68_RS06640 (LVJ68_06630)	1390423..1391412	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ68_RS06645 (LVJ68_06635)	1391488..1391748	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ68_RS06650 (LVJ68_06640)	1391745..1392176	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS06655 (LVJ68_06645)	1392199..1393887	-	1689	WP_003910308.1	PE family protein	-
LVJ68_RS06660 (LVJ68_06650)	1394067..1394927	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ68_RS06665 (LVJ68_06655)	1395008..1395838	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ68_RS06670 (LVJ68_06660)	1395895..1396188	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ68_RS06675 (LVJ68_06665)	1396185..1396454	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228742 WP_003406304.1 NZ_CP089773:1391745-1392176 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228742 NZ_CP124341:c605509-605045 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228742 WP_003406302.1 NZ_CP089773:1391488-1391748 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228742 NZ_CP124341:c605844-605509 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6