Detailed information of TA system TA228741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1245583..1246151
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ68_RS05970
• Protein ID: WP_003405865.1
• Coordinates: 1245777..1246151 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ68_RS05965
• Protein ID: WP_003405863.1
• Coordinates: 1245583..1245780 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS05945 (LVJ68_05940)	1241624..1242262	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ68_RS05950 (LVJ68_05945)	1242352..1243359	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ68_RS05955 (LVJ68_05950)	1243376..1244359	-	984	WP_003898731.1	hypothetical protein	-
LVJ68_RS05960 (LVJ68_05955)	1244422..1245495	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ68_RS05965 (LVJ68_05960)	1245583..1245780	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ68_RS05970 (LVJ68_05965)	1245777..1246151	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS05975 (LVJ68_05970)	1246354..1247052	+	699	WP_003898733.1	hypothetical protein	-
LVJ68_RS05980 (LVJ68_05975)	1247170..1247355	+	186	WP_003901093.1	hypothetical protein	-
LVJ68_RS05985 (LVJ68_05980)	1247282..1247557	-	276	WP_003405867.1	hypothetical protein	-
LVJ68_RS05990 (LVJ68_05985)	1247800..1248123	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ68_RS05995 (LVJ68_05990)	1248138..1248836	-	699	WP_031646953.1	hypothetical protein	-
LVJ68_RS06000 (LVJ68_05995)	1248870..1249079	+	210	WP_003911400.1	hypothetical protein	-
LVJ68_RS06005 (LVJ68_06000)	1249031..1249801	-	771	Protein_1183	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228741 WP_003405865.1 NZ_CP089773:1245777-1246151 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228741 NZ_CP124341:291052-291579 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228741 WP_003405863.1 NZ_CP089773:1245583-1245780 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228741 NZ_CP124341:290779-291045 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07