Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1236827..1237458 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag LVJ68_RS05915 Protein ID WP_003898728.1
Coordinates 1236827..1237138 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag LVJ68_RS05920 Protein ID WP_003405836.1
Coordinates 1237138..1237458 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS05895 (LVJ68_05890) 1232308..1233732 - 1425 WP_003405805.1 class II fumarate hydratase -
LVJ68_RS05900 (LVJ68_05895) 1233763..1234851 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
LVJ68_RS05905 (LVJ68_05900) 1234907..1235551 + 645 WP_003915494.1 DUF4245 domain-containing protein -
LVJ68_RS05910 (LVJ68_05905) 1235558..1236715 - 1158 WP_003898727.1 AI-2E family transporter -
LVJ68_RS05915 (LVJ68_05910) 1236827..1237138 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
LVJ68_RS05920 (LVJ68_05915) 1237138..1237458 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
LVJ68_RS05925 (LVJ68_05920) 1237468..1239004 + 1537 Protein_1167 carboxylesterase/lipase family protein -
LVJ68_RS05930 (LVJ68_05925) 1239011..1240123 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
LVJ68_RS05935 (LVJ68_05930) 1240133..1240390 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
LVJ68_RS05940 (LVJ68_05935) 1240380..1241627 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
LVJ68_RS05945 (LVJ68_05940) 1241624..1242262 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T228740 WP_003898728.1 NZ_CP089773:c1237138-1236827 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T228740 NZ_CP124341:188541-188648 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT228740 WP_003405836.1 NZ_CP089773:c1237458-1237138 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT228740 NZ_CP124341:c188484-188427 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References