Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1029135..1030108 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LVJ68_RS04885 Protein ID WP_003404756.1
Coordinates 1029608..1030108 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID O05910
Locus tag LVJ68_RS04880 Protein ID WP_003898647.1
Coordinates 1029135..1029611 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS04860 (LVJ68_04855) 1024982..1026253 - 1272 WP_003404747.1 PPE family protein -
LVJ68_RS04865 (LVJ68_04860) 1026268..1026567 - 300 WP_003404748.1 PE family protein -
LVJ68_RS04870 (LVJ68_04865) 1027011..1028792 + 1782 WP_003404749.1 BCCT family transporter -
LVJ68_RS04875 (LVJ68_04870) 1028792..1029046 + 255 WP_003898646.1 hypothetical protein -
LVJ68_RS04880 (LVJ68_04875) 1029135..1029611 + 477 WP_003898647.1 DUF1778 domain-containing protein Antitoxin
LVJ68_RS04885 (LVJ68_04880) 1029608..1030108 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LVJ68_RS04895 (LVJ68_04890) 1030421..1031740 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
LVJ68_RS04900 (LVJ68_04895) 1032028..1032609 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LVJ68_RS04905 (LVJ68_04900) 1032609..1034261 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T228738 WP_003404756.1 NZ_CP089773:1029608-1030108 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T228738 NZ_CP124340:c142279-141974 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17387.98 Da        Isoelectric Point: 11.2467

>AT228738 WP_003898647.1 NZ_CP089773:1029135-1029611 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT228738 NZ_CP124340:c142499-142281 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB A5U0V8

References