Detailed information of TA system TA228737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1019605..1020224
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ68_RS04830
• Protein ID: WP_003404726.1
• Coordinates: 1019790..1020224 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ68_RS04825
• Protein ID: WP_003898641.1
• Coordinates: 1019605..1019784 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS04810 (LVJ68_04805)	1015078..1016658	+	1581	WP_003909313.1	serine hydrolase	-
LVJ68_RS04815 (LVJ68_04810)	1016655..1019048	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ68_RS04820 (LVJ68_04815)	1019321..1019479	+	159	WP_003404720.1	hypothetical protein	-
LVJ68_RS04825 (LVJ68_04820)	1019605..1019784	+	180	WP_003898641.1	antitoxin	Antitoxin
LVJ68_RS04830 (LVJ68_04825)	1019790..1020224	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ68_RS04835 (LVJ68_04830)	1020322..1021095	+	774	WP_003404735.1	VOC family protein	-
LVJ68_RS04840 (LVJ68_04835)	1021160..1021609	+	450	WP_003404738.1	hypothetical protein	-
LVJ68_RS04845 (LVJ68_04840)	1021744..1021974	-	231	WP_003898642.1	hypothetical protein	-
LVJ68_RS04850 (LVJ68_04845)	1022141..1023649	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ68_RS04855 (LVJ68_04850)	1023651..1024889	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T228737 WP_003404726.1 NZ_CP089773:1019790-1020224 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T228737 NZ_CP124339:c4550199-4550026 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT228737 WP_003898641.1 NZ_CP089773:1019605-1019784 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT228737 NZ_CP124339:c4550523-4550227 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5