Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 760034..760826 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag LVJ68_RS03505 Protein ID WP_003403386.1
Coordinates 760389..760826 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ68_RS03500 Protein ID WP_003403381.1
Coordinates 760034..760279 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS03470 (LVJ68_03465) 755054..756559 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ68_RS03475 (LVJ68_03470) 756640..757650 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ68_RS03480 (LVJ68_03475) 758038..758421 - 384 WP_003912919.1 ribonuclease VapC6 -
LVJ68_RS03485 (LVJ68_03480) 758516..758671 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ68_RS03490 (LVJ68_03485) 758747..759463 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ68_RS03495 (LVJ68_03490) 759739..760047 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
LVJ68_RS03500 (LVJ68_03495) 760034..760279 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ68_RS03505 (LVJ68_03500) 760389..760826 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ68_RS03510 (LVJ68_03505) 760823..761077 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ68_RS03515 (LVJ68_03510) 761191..763554 + 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ68_RS03520 (LVJ68_03515) 763617..763943 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ68_RS03525 (LVJ68_03520) 763855..764193 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ68_RS03530 (LVJ68_03525) 764190..764363 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T228734 WP_003403386.1 NZ_CP089773:c760826-760389 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T228734 NZ_CP124339:c4156022-4155735 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT228734 WP_003403381.1 NZ_CP089773:c760279-760034 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT228734 NZ_CP124339:c4155748-4155446 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References