Detailed information of TA system TA228733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 759739..760279
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: LVJ68_RS03495
• Protein ID: WP_003403376.1
• Coordinates: 759739..760047 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: LVJ68_RS03500
• Protein ID: WP_003403381.1
• Coordinates: 760034..760279 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS03470 (LVJ68_03465)	755054..756559	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LVJ68_RS03475 (LVJ68_03470)	756640..757650	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
LVJ68_RS03480 (LVJ68_03475)	758038..758421	-	384	WP_003912919.1	ribonuclease VapC6	-
LVJ68_RS03485 (LVJ68_03480)	758516..758671	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ68_RS03490 (LVJ68_03485)	758747..759463	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LVJ68_RS03495 (LVJ68_03490)	759739..760047	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ68_RS03500 (LVJ68_03495)	760034..760279	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
LVJ68_RS03505 (LVJ68_03500)	760389..760826	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
LVJ68_RS03510 (LVJ68_03505)	760823..761077	-	255	WP_003911263.1	antitoxin VapB7	-
LVJ68_RS03515 (LVJ68_03510)	761191..763554	+	2364	WP_003898547.1	arylsulfatase AtsD	-
LVJ68_RS03520 (LVJ68_03515)	763617..763943	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ68_RS03525 (LVJ68_03520)	763855..764193	+	339	WP_003403405.1	PIN domain-containing protein	-
LVJ68_RS03530 (LVJ68_03525)	764190..764363	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T228733 WP_003403376.1 NZ_CP089773:c760047-759739 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T228733 NZ_CP124339:c4046285-4045971 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT228733 WP_003403381.1 NZ_CP089773:c760279-760034 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT228733 NZ_CP124339:c4046521-4046288 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0