Detailed information of TA system TA228727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701795..702441
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ68_RS03155
• Protein ID: WP_003403137.1
• Coordinates: 701795..702208 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ68_RS03160
• Protein ID: WP_003403139.1
• Coordinates: 702205..702441 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS03135 (LVJ68_03130)	697878..699428	+	1551	WP_003403119.1	MlaD family protein	-
LVJ68_RS03140 (LVJ68_03135)	699480..699872	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ68_RS03145 (LVJ68_03140)	699869..700126	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ68_RS03150 (LVJ68_03145)	700309..701544	-	1236	WP_003403128.1	ATP-binding protein	-
LVJ68_RS03155 (LVJ68_03150)	701795..702208	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS03160 (LVJ68_03155)	702205..702441	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ68_RS03165 (LVJ68_03160)	702545..703051	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ68_RS03170 (LVJ68_03165)	703165..703575	-	411	WP_070892737.1	HAMP domain-containing protein	-
LVJ68_RS03175 (LVJ68_03170)	703559..704254	-	696	WP_206647908.1	response regulator transcription factor	-
LVJ68_RS03180 (LVJ68_03175)	704377..704688	+	312	WP_003403164.1	hypothetical protein	-
LVJ68_RS03185 (LVJ68_03180)	704760..705710	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ68_RS03190 (LVJ68_03185)	705951..706535	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ68_RS03195 (LVJ68_03190)	706537..707247	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T228727 WP_003403137.1 NZ_CP089773:c702208-701795 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T228727 NZ_CP124339:2549206-2550528 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT228727 WP_003403139.1 NZ_CP089773:c702441-702205 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT228727 NZ_CP124339:2548940-2549206 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89