Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 699480..700126 Replicon chromosome
Accession NZ_CP089773
Organism Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LVJ68_RS03140 Protein ID WP_003403122.1
Coordinates 699480..699872 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LVJ68_RS03145 Protein ID WP_003403125.1
Coordinates 699869..700126 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ68_RS03125 (LVJ68_03120) 695142..696668 + 1527 WP_003403112.1 virulence factor Mce family protein -
LVJ68_RS03130 (LVJ68_03125) 696731..697873 + 1143 WP_070892736.1 virulence factor Mce family protein -
LVJ68_RS03135 (LVJ68_03130) 697878..699428 + 1551 WP_003403119.1 MlaD family protein -
LVJ68_RS03140 (LVJ68_03135) 699480..699872 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LVJ68_RS03145 (LVJ68_03140) 699869..700126 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LVJ68_RS03150 (LVJ68_03145) 700309..701544 - 1236 WP_003403128.1 ATP-binding protein -
LVJ68_RS03155 (LVJ68_03150) 701795..702208 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LVJ68_RS03160 (LVJ68_03155) 702205..702441 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LVJ68_RS03165 (LVJ68_03160) 702545..703051 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ68_RS03170 (LVJ68_03165) 703165..703575 - 411 WP_070892737.1 HAMP domain-containing protein -
LVJ68_RS03175 (LVJ68_03170) 703559..704254 - 696 WP_206647908.1 response regulator transcription factor -
LVJ68_RS03180 (LVJ68_03175) 704377..704688 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T228726 WP_003403122.1 NZ_CP089773:c699872-699480 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T228726 NZ_CP124339:2268523-2268625 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT228726 WP_003403125.1 NZ_CP089773:c700126-699869 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT228726 NZ_CP124339:c2268663-2268395 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References