Detailed information of TA system TA228724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 644878..645554
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ68_RS02905
• Protein ID: WP_003898500.1
• Coordinates: 644878..645291 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ68_RS02910
• Protein ID: WP_003402915.1
• Coordinates: 645288..645554 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS02875 (LVJ68_02870)	640223..640525	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ68_RS02880 (LVJ68_02875)	640585..640863	-	279	WP_003402901.1	hypothetical protein	-
LVJ68_RS02885 (LVJ68_02880)	640860..642113	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ68_RS02890 (LVJ68_02885)	642233..642619	-	387	WP_003402907.1	VOC family protein	-
LVJ68_RS02895 (LVJ68_02890)	642682..643566	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ68_RS02900 (LVJ68_02895)	643662..644564	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ68_RS02905 (LVJ68_02900)	644878..645291	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ68_RS02910 (LVJ68_02905)	645288..645554	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ68_RS02915 (LVJ68_02910)	645746..647461	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ68_RS02920 (LVJ68_02915)	647539..649143	+	1605	WP_003402920.1	amidohydrolase	-
LVJ68_RS02925 (LVJ68_02920)	649140..650120	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T228724 WP_003898500.1 NZ_CP089773:c645291-644878 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T228724 NZ_CP124339:2012411-2012665 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT228724 WP_003402915.1 NZ_CP089773:c645554-645288 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT228724 NZ_CP124339:2012163-2012414 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0