Detailed information of TA system TA228721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 367952..368523
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ68_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 367952..368254 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: LVJ68_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 368302..368523 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS01575 (LVJ68_01575)	363421..364224	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ68_RS01580 (LVJ68_01580)	364234..365631	-	1398	WP_003401544.1	sulfatase	-
LVJ68_RS01585 (LVJ68_01585)	365810..367585	+	1776	WP_010886074.1	PE family protein	-
LVJ68_RS01590 (LVJ68_01590)	367728..367955	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
LVJ68_RS01595 (LVJ68_01595)	367952..368254	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ68_RS01600 (LVJ68_01600)	368302..368523	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
LVJ68_RS01605 (LVJ68_01605)	368520..368945	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ68_RS01610 (LVJ68_01610)	369081..369713	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ68_RS01615 (LVJ68_01615)	369710..370618	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ68_RS01620 (LVJ68_01620)	370626..371216	-	591	WP_229298012.1	hypothetical protein	-
LVJ68_RS01625 (LVJ68_01625)	371209..371259	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T228721 WP_003401560.1 NZ_CP089773:367952-368254 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T228721 NZ_CP124339:c827241-826864 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT228721 WP_003401563.1 NZ_CP089773:368302-368523 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT228721 NZ_CP124339:c827698-827318 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A829CG48